光动力过程中线粒体膜电位和细胞存活关系

以1-anilionaphthalene-8-sulfonate(ANS)作荧光探针，通过其荧光光谱研究了苯硫基酞菁锌PcS)、苯硫基铝酞菁(AIPcS)和烷氧基铝酞菁(AIPc)这三种金属酞菁配合物作为光敏剂的光动力作用对癌细胞线粒体膜表面电位的影响．研究表明，光动力作用后线粒体膜表面电位降低，表面电荷数面密度增加．ZnPcS的影响最大，这与酶联免疫检测光动力作用后对癌细胞的杀伤效果相一致，提示细胞线粒体膜可能是金属酞菁配合物在光动力过程中的作用位点。通过比较细胞线粒体膜表面电位以及表面电荷数面密度与细胞存活之间的关系，阐述了光动力作用的物理学机制．同时，由于线粒体膜电位与细胞凋亡的密切关系，金属酞菁配合物对线粒体膜表面电位的影响提供了一个衡量药物疗效的判据。

RELATIONSHIP BETWEEN MITOCHONDRIAL MEMBRANE POTENTIAL AND CELL LIVABILITY IN PHOTODYNAMIC THERAPY

With ANS as fluorescence probe, effect of three metal phthalocyanines on mitochondrial membrane surface potential and charge density in photodynamic therapy were investigated; meanwhile, cell survival was studied by MTT. Results showed that effect of ZnPcS was most prominent and its effect on mitochondrial membrane surface potential was coincident with the result of MTT. The mitochondrial membrane may be the binding site of photosensitizers in PDT. Because of the close relationship between mitochondrial membrane potential and cell apoptosis, the effect of photosensitizers on mitochondrial membrane surface potential proves an evidence for its curative effect, and the physical mechanism in PDT was discussed.