Radioimmunoassay of estrone and estradiol-17 in peripheral plasma of pregnant and non-pregnant women |
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| Authors: | R de Hertogh |
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| Affiliation: | 1. Department of Biostatistics and Epidemiology, University of Pennsylvania, 8th floor Blockley Hall, 423 Guardian Drive, Philadelphia, PA 19104-6021, USA;2. Department of Kinesiology, The Penn State University, 276 Recreation Building, University Park, PA 16802, USA;3. Department of Radiology, University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104, USA;4. Department of Food Science and Nutrition, 225 FScN, 1334 Eckles Ave, University of Minnesota, St. Paul, MN 55108, USA;5. Department of Medicine, Division of Medical Oncology, University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, USA;6. Department of Physical Therapy, Richard Stockton College of New Jersey, 101 Vera King Farris Drive, Galloway, NJ 08205-9441, USA;7. Department of Radiology, University of Pittsburgh, 3352 5th Ave, Room 130, Pittsburgh, PA 15213, USA |
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| Abstract: | Estrone and estradiol-17β have been measured in non-pregnancy plasma, by radioimmunoassay, after Chromatographie separation on short Sephadex LH20 columns. Dextran coated charcoal has been used for adsorbing unbound estrogen in the incubation mixture. No meaningful results could be obtained by measurement of total free estrogens in crude extracts of non-pregnancy plasma.In non-pregnancy plasma, the limited specificity of the antibodies imposed the chromatography step. The precision of the measurements during the menstrual cycle was of 9–15% (coefficient of variation).In pregnancy plasma the direct measurement of free estrogens in crude extracts, without chromatography, gave results that were proportional to the volume of plasma extracted.The precision of the measurements varied between 7–15% (coefficient of variation), for both low and high plasma concentration. In pregnant subjects a good correlation was found between the level of unconjugated estrogens in the plasma and the level of urinary estriol. |
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