In vitro effect of various mitogens on starfish (Asterias rubens) axial organ cells |
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Authors: | C Brillouet M Leclerc J Panuel R Binaghi |
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Institution: | 1. Département de Biologie, Université d''Orléans 45045, Orleans Cedex, France;2. Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France;3. Collège de France, Place Marcelin Berthelot, 75005 Paris, France |
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Abstract: | The effects of various mitogens on axial organ (AO) cells of the sea star have been studied. Pokeweed mitogen (PWM) stimulates 3H]thymidine incorporation by the whole population of axial organ cells of the sea star. This effect occurs 24 hr after the addition of PWM and is maximal at 40 μg/ml. In contrast, no stimulation is observed when coelomocytes are treated with PWM under the same conditions. No stimulation of the whole AO cell population is observed in the presence of Con A or LPS. However, the AO cell population can be divided, on the basis of surface adherence properties, into two subpopulations, adherent and nonadherent. Con A stimulates the nonadherent cells, but not the adherent cells: The stimulating effect is maximal 24 hr after addition of Con A and at 0.2–0.5 μg/ml. In contrast, LPS stimulates the adherent but not the nonadherent cells and the stimulating effect is maximal at 24 hr and at 45 μg/ml. |
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Keywords: | To whom correspondence should be addressed |
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