Enhanced proliferation,survival, and yield of cultured myelin basic protein (MBP)-reactive Lewis rat lymph node cells following dual activation with concanavalin A and MBP |
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Authors: | Haim Ovadia Philip Y Paterson |
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Institution: | Department of Microbiology-Immunology, The Medical and Dental Schools, Northwestern University, Chicago, Illinois 60611 USA |
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Abstract: | Lymph node cells (LNC) from Lewis rats sensitized 7 days previously to myelin basic protein (MBP) exhibit greatly increased survival and undergo much greater proliferation when cultured in the presence of both concanavalin A (Con A) and MBP. Dual activation, in contrast to culture with either the mitogen or the neural antigen, results in a substantially greater yield of lymphoblasts with the capacity to transfer experimental allergic encephalomyelitis (EAE) to normal syngeneic recipients. Dual activation provides a practical advantage over other methods reported to date for securing larger numbers of functional MBP-reactive lymphoid cells for immunologic studies. |
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