Augmentation of mouse natural killer cell activity by muramyl dipeptide and its analogs |
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Authors: | Somesh D Sharma Van Tsai James L Krahenbuhl Jack S Remington |
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Institution: | 1. Division of Allergy, Immunology and Infectious Diseases, Palo Alto Medical Research Foundation, Palo Alto, California 94301 U.S.A.;2. Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California 94305 U.S.A. |
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Abstract: | The ability of muramyl dipeptide (MDP) and its structural analogs (des-MDP, abu-MDP, and des-abu-MDP) to influence mouse natural killer (NK) cells in two different strains of mice was examined. In CBA/J mice, administration of MDP by both intraperitoneal (ip) and intravenous (iv) routes enhanced splenic NK cell activity. Maximum augmentation of NK cell activity was observed 3 days after MDP treatment. NK cell activity was also stimulated upon in vitro culture of CBA/J mouse spleen cells with MDP. Only iv inoculation of MDP to C57BL/6 mice 7 days previously enhanced NK cell activity of spleen cells. Peritoneal NK cell activity was not affected in either strain of mice, regardless of the route of inoculation of MDP. Two structural analogs of MDP, abu-MDP and des-abu-MDP, enhanced peritoneal NK cell activity, whereas des-MDP had no effect when tested 3 days after ip treatment of CBA/J mice with these compounds. Peritoneal NK cell activity of C57BL/6 mice was not modulated by des-MDP, abu-MDP, or des-abu-MDP. A synergistic effect on peritoneal NK cell activity was observed in both CBA/J and C57BL/6 mice treated first with MDP and then with lipopolysaccharide (LPS) or Bacillus Calmette-Guerin (BCG). |
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Keywords: | Address reprint requests to: Somesh D Sharma Ph D Palo Alto Medical Research Foundation 860 Bryant Street Palo Alto Calif 94301 |
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