Fractionation of murine macrophage inhibitory factor into two distinct species |
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| Authors: | Daniel Bout Alice L. Kühner Volker Klimetzek Heinz G. Remold John R. David |
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| Affiliation: | 1. Departments of Medicine, Harvard Medical School Boston, Massachusetts 02115 U.S.A.;2. The Robert B. Brigham Division of the Affiliated Hospitals Center, Inc., Boston, Massachusetts 02115 U.S.A. |
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| Abstract: | Murine migration inhibitory factor (MIF) produced by concanavalin A-stimulated lymph node cells from C57BL/6 mice was fractionated by Sephadex G-100 gel filtration, density gradient electrophoresis, and isoelectrofocusing in a sucrose density gradient and assayed on in vitro-cultivated bone marrow macrophages from C57BL/6 mice. Two major MIF species, pH3-MIF with an isoelectric point of 3.0–4.3 and pH5-MIF with an isoelectric point of 4.6 to 5.2, were obtained. The similarity of murine MIF to guinea pig and human MIF is discussed. |
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| Keywords: | To whom all correspondence should be addressed. |
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