A study of the ability of H-2Kk-Iak containing subcellular fractions to elicit primary anti-H-2 cytotoxic T lymphocytes |
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Authors: | Arthur H. Hale Donald L. Evans Maria P. McGee |
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Affiliation: | Department of Microbiology and Immunology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103 U.S.A. |
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Abstract: | In order to identify an antigen required for elicitation of anti-H-2 cytotoxic T lymphocytes (CTLs), we have purified the H-2-Kk glycoprotein, incorporated it into a lipid vesicle, and tested it for its ability to elicit anti-H-2Kk CTLs. The results indicate that a lipid vesicle containing purified H-2Kk can elicit specific secondary anti-H-2Kk CTLs. In addition it was shown that in association with a partially purified Iak fraction the primary anti-H-2Kk CTL response was enhanced. It was also shown that Iak antigens alone could elicit an anti-H-2k CTL response. Although a low primary response was found with purified H-2Kk, it was observed that lipid vesicles containing both H-2Kk and Iak glycoproteins could elicit a significantly enhanced primary anti-H-2Kk CTL response. Lipid vesicles containing H-2Kk-Iak were tested for their enhanced capacity to elicit anti-H-2 CTLs as well as for their ability to elicit anti-H-2Kk CTLs in the presence of supernatants from concanavalin A-stimulated spleen cells. |
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Keywords: | To whom correspondence should be sent. |
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