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High resolution bands in maize chromosomes by G-banding methods |
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| Authors: | K. Kakeda H. Yamagata K. Fukui M. Ohno K. Fukui Z. Z. Wei E. S. Zhu |
| Affiliation: | (1) Department of Molecular Biology, National Institute of Agrobiological Resources, Kannondai, 305 Tsukuba, Japan;(2) Kyoto University, 606 Kyoto, Japan;(3) Toho University, 274 Funahashi, Japan;(4) Chinese Academy of Agricultural Sciences, Institute of Crop Breeding and Cultivation, Beijing, China;(5) Present address: Kyoto University, 606 Kyoto, Japan;(6) Present address: Plant Biotechnology Department, Mitsui Toatsu Cemicals, Nishikubo, 253 Chigasaki, Japan |
| Abstract: | Summary It was demonstrated that G-bands are unequivocally present in plant chromosomes, in contrast to what had been formerly believed by plant cytologists. Maize chromosomes prepared by an enzymatic maceration method and treated with trypsin or SDS showed clear G-bands spreading along the chromosomes. The most critical point during the G-banding procedures was the post-fixation with glutaraldehyde solution. Banding patterns were processed by using the chromosome image analyzing system and a clearer image was obtained. Gbanding technique and the image manipulation method described here can be applied to many plant species, and would contribute new information in the field of plant cytology and genetics. |
| Keywords: | Maize Chromosome banding G-banding method Enzymatic maceration Image analysis |
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