High sensitivity of DNA replication to 5-aminouracil in the middle of the S period |
| |
Authors: | Matilde H Navarrete Gardenia González-Gil S Martín-Hurtado J F López-Sáez |
| |
Institution: | (1) Instituto de Biologia Celular, Velázquez 144, Madrid-6, Spain |
| |
Abstract: | Summary Cell distribution in different compartments of the cell cycle (G1, early, middle and late S, G2 and mitosis) has been studied during treatment with 0.5 mM 5-aminouracil and recovery inAllium cepa L. root meristems by cytophotometric and autoradiographic methods. At optimum conditions for obtaining mitotic synchronization, 5-aminouracil gives rise to cell accumulation in the S period, preferentially in its middle zone where the relative DNA content is 2.8 ± 0.1 C. After a 14-hour treatment 33% of the proliferative population is accumulated in this particular region.During recovery, a drastic reduction of the S phase and a clear increase of the mitotic frequency are the most important events observed. Apparently, the removal of the drug frees the blockage and the accumulated cells complete their interphase making up the mitotic wave. |
| |
Keywords: | Allium cepa 5-aminouracil DNA replication Interphase accumulation Mitotic synchrony |
本文献已被 SpringerLink 等数据库收录! |
|