Determination of 6-hydroxychlorzoxazone and chlorzoxazone in porcine microsome samples |
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| Authors: | Cox Sherry K Hamner Tina Bartges Joe |
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| Institution: | Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, 37901, Knoxville, TN, USA. scox6@utk.edu |
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| Abstract: | A simple, accurate and sensitive HPLC method for the in vitro determination of 6-hydroxychlorzoxazone and chlorzoxazone in porcine microsome samples is described. Chromatography was performed on a YMC-Pack ODS-AQ column using a mobile phase of 0.05% phosphoric acid pH 3-methanol (60:40, v/v). UV detection was carried out at 287 nm. The detector response was linear over the concentration range 25-2000 ng/ml. This assay produced quick, accurate, and repeatable results. |
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| Keywords: | Hydroxychlorzoxazone Chlorzoxazone |
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