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| 从土壤中提取DNA用于PCR扩增 | |
| 引用本文： | 杜涛,黄小毛,侯明生,林木兰,周宁一. 从土壤中提取DNA用于PCR扩增[J]. 微生物学通报, 2003, 30(6): 1-5 |
| 作者姓名： | 杜涛 黄小毛 侯明生 林木兰 周宁一 |
| 作者单位： | 1. 中国科学院武汉病毒研究所,武汉,430071 2. 中国科学院武汉病毒研究所,武汉,430071;华中农业大学植物科技学院,武汉,430070 3. 华中农业大学植物科技学院,武汉,430070 |
| 基金项目： | 国家自然科学基金(No.3017074) |
| 摘 要： | 设计、比较了5种直接从土壤中提取DNA的方法。实验结果表明这5种方法都可以从土壤中提取到长度大于15kb的DNA片段，但在不同方法间DNA的产量存在很大差异；初提的土壤DNA经进一步提纯后均可用于PCR反应，利用细菌16S rRNA基因和抗菌肽Shiva-1基因的引物都得到了相应的目的产物。其中方法5提取DNA产量最高，无明显降解，且重复性好，是一种从小量土壤样品中直接提取DNA的理想方法。 |
| 关 键 词： | 土壤 DNA 提取方法 PCR |
| 文章编号： | 0253-2654(2003)06-0001-05 |
| 修稿时间： | 2003-01-07 |
EXTRACTION OF DNA FROM SOIL FOR PCR AMPLIFICATION |
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| DU Tao HUANG Xiao-Mao HOU Ming-Sheng LIN Mu-Lan ZHOU Ning-Yi. EXTRACTION OF DNA FROM SOIL FOR PCR AMPLIFICATION[J]. Microbiology China, 2003, 30(6): 1-5 | |
| Authors: | DU Tao HUANG Xiao-Mao HOU Ming-Sheng LIN Mu-Lan ZHOU Ning-Yi |
| Abstract: | In this paper, five different methods were carried out for DNA extraction directly from soil. The result shows that all five methods could generate DNA with more than 15 kb in size. They were subsequently used as templates for PCR amplification with success, using primers of the bacterial 16S rRNA gene and Shiva-1 gene encoding an antibacterial peptide. However, method 5 is more suitable for DNA extraction directly from a small amount of soil sample as it produced a good yield of DNA in high integrity with reliable reproducibility. |
| Keywords: | Soil DNA Extaction PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |