| 融合基因Mdc-hly的构建及原核表达 |
| |
| 引用本文： | 卢雪梅,金小宝,朱家勇,梅寒芳,马艳,王艳,李小波,褚夫江.融合基因Mdc-hly的构建及原核表达[J].生物技术通报,2010(4). |
| |
| 作者姓名： | 卢雪梅 金小宝 朱家勇 梅寒芳 马艳 王艳 李小波 褚夫江 |
| |
| 作者单位： | 广东药学院基础学院,广东省生物活性药物研究重点实验室,广州,510006 |
| |
| 基金项目： | 国家自然科学基金项目 |
| |
| 摘 要： | 构建家蝇天蚕素-人溶菌酶(Mdc-hly)融合基因,实现Mdc-hly基因在大肠杆菌中的表达。通过RT-PCR分别扩增出家蝇天蚕素和人溶菌酶的成熟肽基因序列,再利用Gene-SOEing技术构建融合基因,将融合基因克隆至pET32a表达载体,转化E.coli BL21(DE3),经IPTG诱导得到高效表达,融合蛋白分子量约为38kD。Western blotting杂交证实了表达蛋白的抗原活性。成功构建了融合其因并进行了原核表达,为进一步的生物活性研究打下基础。
|
| 关 键 词： | 家蝇天蚕素 人溶菌酶 原核表达 |
Construction and Prokaryotic Expression of Fusion Gene Mdc-hly |
| |
| Lu Xuemei,Jin Xiaobao,Zhu Jiayong,Mei Hanfang,Ma Yan,Wang Yan,Li Xiaobo,Chu Fujiang.Construction and Prokaryotic Expression of Fusion Gene Mdc-hly[J].Biotechnology Bulletin,2010(4). |
| |
| Authors: | Lu Xuemei Jin Xiaobao Zhu Jiayong Mei Hanfang Ma Yan Wang Yan Li Xiaobo Chu Fujiang |
| |
| Institution: | Lu Xuemei Jin Xiaobao Zhu Jiayong Mei Hanfang Ma Yan Wang Yan Li Xiaobo Chu Fujiang(School of Basic Courses,Guangdong University of Pharmaceutical,Guangdong Key Laboratory of Bioactive Drugs,Guangzhou 510006) |
| |
| Abstract: | The DNA fragment encoding the mature peptide of Musca domestica cecropin and human lysozyme were obtained by RT-PCR. The Mdc-hly gene was constructed in a Mdc-linker-hly format with the standard 15-amino acid linker (Gly4Ser)3 by Gene-SOEing,and the final full length product was cloned into the pET-32a vector for protein expression in Escherichia coli strain BL21(DE3). The expression fusion protein Mdc-hly-Trx were about 38 kD and Western blotting analysis confirmed that the 38 kD protein was the fusion pro... |
| |
| Keywords: | Gene-SOEing Musca domestica cecropin Human lysozyme Gene-SOEing Prokaryotic expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
