Phosphate-dependent sodium transport in S. faecalis investigated by 23Na and 31P NMR |
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| Authors: | F Rabaste G Dauphin G Jeminet J Guyot A M Delort |
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| Affiliation: | Université Blaise Pascal Clermont-Ferrand, Laboratoire de Chimie Organique Biologique-U.R.A., 485 du C.N.R.S., Aubière, France. |
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| Abstract: | Na+ movements in S. faecalis were studied by 23Na NMR. They proved to be dependent on phosphate concentration in the buffer during the de-energization step. K+ and H+ were also studied respectively by potentiometry and 31P NMR and were shown not to be implicated. For de-energized cells the internal phosphate concentration, on the contrary, was directly linked to the external phosphate contained in the buffer. The experiments showed a Na+/Pi dependence in this prokaryote so far known only in eukaryotes. |
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