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Mineral and Carbohydrate Nutrition of Plant Cell and Tissue Cultures |
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| Authors: | Carlo Leifert Kenneth P Murphy Peter J Lumsden |
| Institution: | 1. Department of Plant and Soil Science , University of Aberdeen , Cruickshank Building St. Machar Drive, Aberdeen , AB9 2UD , Scotland, UK;2. Department of Applied Biology , University of Central Lancashire , Preston , PR1 2HE , England, UK |
| Abstract: | Recent advances in our understanding of the mineral (and carbohydrate) nutrition of cultured plant cells and tissues are reviewed. The methods used for empirical selection of nutrient composition of culture media for different plant genera/species and types of culture are critically evaluated. The acquisition of nutrients is discussed in terms of their physical availability in the culture medium and uptake from the medium. The effect on uptake of factors such as pH and water potential and the relationship with growth rates and medium depletion are examined in detail. Finally, some effects of nutrients on morphogenesis of plants are reviewed. |
| Keywords: | plant tissue culture nutrition carbohydrates nutrient uptake micropropagation nutrient analysis organogenesis pH humidity |