A novel type of somatic embryogenesis in Coffea arabica |
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Authors: | Beat Neuenschwander Thomas W Baumann |
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Institution: | (1) Institute of Plant Biology, University of Zurich, CH-8008 Zurich, Switzerland |
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Abstract: | A novel type of somatic embryogenesis characterized by an efficient and highly synchronized embryo formation was observed in embryogenic callus of Coffea arabica initiated on Murashige and Skoog medium containing kinetin (4 mg/l) and 2,4-D (1 mg/l). It occurs in suspension and goes along with the suppression of High Frequency Somatic Embryo Induction (HFSE). This is achieved by favoring during cultivation senescence-or necrosis-like processes which apparently do not impair the competence for embryogenesis. Since the resulting embryos germinate at a rate of 94.5 % without the need of a maturation step, we propose the term Self-Controlled Somatic Embryogenesis (SCSE).In addition, HFSE was optimized using half-strength liquid medium with 0.1 mg/l kinetin and 0.25 mg/l 2,4-D for proliferation of embryonic tissue, and 2.6 mg/l ABA for maturation of embryos. Yields as well as germination rates of HFSE embryos were markedly lower as compared to SCSE.Abbreviations ABA
abscisic acid
- BAP
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxy-acetic acid
- NAA
1-naphthaleneacetic acid
- HFSE
high frequency somatic embryo induction
- LFSE
low frequency somatic embryo induction
- SCSE
self-controlled somatic embryogenesis |
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Keywords: | Somatic embryogenesis plant regeneration cell suspension coffee Coffea arabica |
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