NMR confirmation of an alkali-insoluble glucan from Kluyveromyces marxianus cultivated on a lactose-based medium |
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Authors: | Lukondeh Tredwell Ashbolt Nicholas J Rogers Peter L Hook James M |
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Institution: | (1) School of Civil and Environmental Engineering, The University of New South Wales, UNSW, Sydney, 2052, Australia; Tel.: |
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Abstract: | Yeast cells of Kluyveromyces marxianus grown in a sterile lactose-based medium, were extracted with sodium hydroxide to give a fraction of alkali-insoluble yeast (AIY) glucan. From 1H- and 13C-NMR characterization it was concluded that the glucan consisted mostly of (1 3) linkages and was similar to that obtained from Saccharomyces cerevisiae used in the preparations for skin revitalization and repair. The molecular weight of the glucan was determined to be approximately 3 × 105 Da. Therefore, K. marxianus grown on a lactose-based medium similar to whey, a waste stream of the dairy industry, could be a commercial source of (1 3) glucans for use in the food and pharmaceutical industries. |
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Keywords: | Glucan lactose NMR solid state yeast |
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