Endothelin-1- and endothelin-3-induced vasorelaxation via common generation of endothelium-derived nitric oxide. |
| |
Authors: | A Namiki Y Hirata M Ishikawa M Moroi J Aikawa K Machii |
| |
Affiliation: | Third Department of Internal Medicine, Toho University School of Medicine, Tokyo, Japan. |
| |
Abstract: | The vasorelaxant effects by endothelin-1 (ET-1) and endothelin-3 (ET-3), and their mechanisms of action were studied in isolated porcine pulmonary arterial strips. ET-1 and ET-3 dose-dependently (10(-9) - 10(-8) M) relaxed vascular strips precontracted with norepinephrine only in the presence of endothelium. The maximal vasorelaxant effect by ET-1 was about 70% of that by ET-3. The ET-1- and ET-3- induced vasorelaxation was blocked by NG-nitro-L-arginine, an inhibitor of nitric oxide synthesis, and methylene blue, an inhibitor of soluble guanylate cyclase. The present data suggest that vascular smooth muscle relaxation induced by ET-1 and ET-3 is mainly ascribed to synthesis and release of nitric oxide from L-arginine in endothelium. |
| |
Keywords: | |
|
|