Evaluation of the Biolog system for the identification of Bacillus anthracis |
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| Authors: | LWJ Baillie MN Jones PCB Turnbull RJ Manchee |
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| Institution: | Defence Microbiology Division, Chemical and Biological Defence Establishment, Porton Down;Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, UK |
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| Abstract: | The potential of the Biolog system for the identification of Bacillus anthracis was evaluated. In-house generated databases allowed the correct identification of 19 of 20 isolates of B. anthracis within 24 h. Five strains of the closely related B. cereus/thuringiensis group were misidentified as B. anthracis. For this reason the test could only serve as a primary screen with further testing being required to confirm identity. In addition 20% of all the strains of bacilli examined during the study gave unreadable reaction profiles due to false-positive reactions. |
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