Isolation and characterization of a DNA probe for Staphylococcus aureus subspecies aureus biovar |
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Authors: | Fontana C Favaro M Frezza D Favalli C |
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Affiliation: | Department of Experimental Medicine and Biochemical Science, University of Rome Tor Vergata, Italy. fontanac@uniroma2.it |
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Abstract: | The gene encoding for polynucleotide phosphorylase (pnp) of a new biovar of Staphylococcus aureus subsp. aureus (NBSA) has been isolated from a genomic library of strain M280(0). The coding region consisted of a 1094-bp HindIII-HindIII DNA fragment encoding for a protein of 277 amino acids with a calculated molecular mass of 29.5 kDa. The nucleotide sequence of the structural gene, contained a continuous open reading frame of 836 bp, showed significant homology with the genes of bacterial polynucleotide phosphorylase from Bacillus subtilis (67.7% identity), from Haemophilus influenzae (62.4% identity), from Pseudomonas luminescens (61.6% identity), and from Escherichia coli (59.7% identity). DNA-DNA and DNA-colony slot-blot hybridizations demonstrated that the pnp gene, employed as a molecular probe, is specific for the identification of NBSA strains. |
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Keywords: | Staphylococcus spp. Molecular cloning Sequence Hybridization probe |
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