The translation of rabbit hemoglobin messenger RNA in a cell-free extract from chick embryo brain |
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Authors: | Don Hendrick Walter Knöchel Walter Schwarz Sabine Pitzel Heinz Tiedemann |
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Affiliation: | Institut für molekularbiologie und Biochemie der Freien Universität Berlin, 1 Berlin 33, Arnimallee 22, Germany |
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Abstract: | The translation of rabbit hemoglobin messenger RNA in an unfractionated cytoplasmic extract from chick embryo brain was studied. This translation was not dependent upon reticulocyte-specific factors. An analysis of the product synthesized in vitro with the embryo brain cell-free extract and rabbit hemoglobin messenger RNA by carboxymethyl cellulose chromatography showed that the system was capable of synthesizing both the α and β globin chains. Analysis of the tryptic peptides of the in vitro synthesized α chain by ion-exchange chromatography showed that the embryo brain extract with rabbit hemoglobin messenger RNA was capable of synthesizing the complete α chain of rabbit hemoglobin. The results suggest that no stringent tissue-specific controls exist for the translation of globin messenger RNA and were discussed in this context. |
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