Thermoresponsive microtubule hydrogel with high hierarchical structure |
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Authors: | Sano Ken-Ichi Kawamura Ryuzo Tominaga Taiki Nakagawa Hiromichi Oda Naoko Ijiro Kuniharu Osada Yoshihito |
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Affiliation: | Molecular & System Life Science Unit, Advanced Science Institute, RIKEN, Saitama, Japan. |
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Abstract: | A thermoresponsive 3D microtubule hydrogel (MT gel) was prepared by simultaneous polymerization and chemical cross-linking of tubulins. The main chain of this gel is composed of cross-linked MTs, which consists of a cylindrical assembly of tubulin covalently connected by polyethylene glycol. This gel, which contains 10 mg/mL of tubulin, exhibits a storage modulus G' as high as 1 × 10(3), which is 10 times higher than the loss modulus G' over a wide range of frequencies. The MT gel exhibits a reversible sol-gel transition by temperature changes at 4-37 °C via depolymerization and polymerization of the MT network. Notable effects of the presence of the cross-linkage on the process of polymerization and depolymerization of tubulin were experimentally observed, and the role of the cross-linkage was discussed. |
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