首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Hair follicular expression and function of group X secreted phospholipase A2 in mouse skin
Authors:Yamamoto Kei  Taketomi Yoshitaka  Isogai Yuki  Miki Yoshimi  Sato Hiroyasu  Masuda Seiko  Nishito Yasumasa  Morioka Kiyokazu  Ishimoto Yoshikazu  Suzuki Noriko  Yokota Yasunori  Hanasaki Kohji  Ishikawa Yukio  Ishii Toshiharu  Kobayashi Tetsuyuki  Fukami Kiyoko  Ikeda Kazutaka  Nakanishi Hiroki  Taguchi Ryo  Murakami Makoto
Institution:Lipid Metabolism Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Abstract:Although perturbed lipid metabolism can often lead to skin abnormality, the role of phospholipase A(2) (PLA(2)) in skin homeostasis is poorly understood. In the present study we found that group X-secreted PLA(2) (sPLA(2)-X) was expressed in the outermost epithelium of hair follicles in synchrony with the anagen phase of hair cycling. Transgenic mice overexpressing sPLA(2)-X (PLA2G10-Tg) displayed alopecia, which was accompanied by hair follicle distortion with reduced expression of genes related to hair development, during a postnatal hair cycle. Additionally, the epidermis and sebaceous glands of PLA2G10-Tg skin were hyperplasic. Proteolytic activation of sPLA(2)-X in PLA2G10-Tg skin was accompanied by preferential hydrolysis of phosphatidylethanolamine species with polyunsaturated fatty acids as well as elevated production of some if not all eicosanoids. Importantly, the skin of Pla2g10-deficient mice had abnormal hair follicles with noticeable reduction in a subset of hair genes, a hypoplasic outer root sheath, a reduced number of melanin granules, and unexpected up-regulation of prostanoid synthesis. Collectively, our study highlights the spatiotemporal expression of sPLA(2)-X in hair follicles, the presence of skin-specific machinery leading to sPLA(2)-X activation, a functional link of sPLA(2)-X with hair follicle homeostasis, and compartmentalization of the prostanoid pathway in hair follicles and epidermis.
Keywords:Eicosanoid  Mouse  Phospholipase A  Phospholipid  Skin
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号