Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method |
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Authors: | Kubareva E A Walter J Karyagina A S Vorob'eva O V Lau P C K Trautner T |
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Institution: | Institute of Physico-Chemical Biology, Moscow State University, Russia. kubareva@belozersky.msu.ru |
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Abstract: | Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pentanucleotide recognition sequence 5'-CCNGG-3' (where N = any nucleoside). 5-Methylcytosine (m5C) type modification by M-N1aX was confirmed by the use of oligonucleotide substrates that contain 5-fluoro-2'-deoxycytidine. |
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