Pulse discharge of calcium through a demoralizing epithelium in the crustacean Orchestia: Ultrastructural cytochemistry and X-ray microanalysis |
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| Institution: | 1. Laboratoire de Biologie animale et générale. Facultédes Sciences de la Vie et de l''Environnement, 6, Boulevard Gabriel 21100 Dijon, France;2. Laboratoire de Cytologie Expérimentale, Facultédes Sciences, Parc Valrose, 06034 Nice, France;1. Centre for Medical Image Computing (CMIC), Department of Medical Physics and Biomedical Engineering, University College London, United Kingdom;2. BCBL. Basque Center on Cognition, Brain and Language, Spain;3. Human Neuroanatomy Laboratory, University of Castilla-La Mancha, Spain;4. Dementia Research Centre, Department of Neurodegenerative Disease, Institute of Neurology, University College London, United Kingdom;5. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, USA;6. Department of Applied Mathematics and Computer Science, Technical University of Denmark, Denmark;7. MIT Computer Science and Artificial Intelligence Laboratory, USA;1. Institute for Research, Development and Innovation, International Medical University, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia;2. School of Postgraduate Studies, International Medical University, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia;3. School of Pharmacy, International Medical University, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia;1. Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650093, China;2. Department of Materials Science & Engineering, North Carolina State University, Raleigh, NC 27695, USA;3. School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China;1. Department of Surgery, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 210-711, Republic of Korea;2. College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea;3. Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea;4. Natural Product Research Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea;5. Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Sungnam Gyeonggi-do 461-701, Republic of Korea;6. Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea;1. Centre de transfusion sanguine de Polynésie française, hôpital du Taaone, centre hospitalier de Polynésie française, Pirae, BP 4530, 98713 Papeete, Tahiti, French Polynesia;2. Laboratoire de biologie, hôpital du Taaone, centre hospitalier de Polynésie française, Pirae, Tahiti, French Polynesia;3. Unité d’hémovigilance, hôpital du Taaone, centre hospitalier de Polynésie française, Pirae, Tahiti, French Polynesia;4. Institut national de la transfusion sanguine, département des agents transmissibles par le sang, centre national de référence risques infectieux transfusionnels, Paris, France |
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| Abstract: | During the post-exuvial period, the posterior caeca of the terrestrial crustacean Orchestia quickly reabsorb the calcareous concretions stored during the pre-exuvial period through successive generations of spherules which appear, grow, then disappear from the apex to the base of a typical network of extracellular channels. The caecal epithelium is thus alternatively calcium-loaded and unloaded. Ultrastructural cytochemistry, using direct precipitating methods (potassium pyroantimonate, oxalic acid and sodium fluoride) or indirect substitution method (lead acetate) reveals that this extracellular pathway could be the main route for a massive transport down a concentration gradient of ionic or ionizable calcium which temporarily precipitates into instable spherules basally solubilized. Quantitative microanalysis of both frozen dry sections and anhydrous thin sections of cryosubstituted resin-embedded material indicates maximal paracellular calcium concentrations during the loading phases, which may be responsible not only for the calcification of the spherules but also for the abnormally high calcium content within the cytoplasm and the mitochondria. |
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