Isolation,sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation |
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Authors: | Jean-Philippe Galaud Jean-Jacques Lareyre Nicole Boyer |
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Institution: | (1) U.A. Physiologie Intégree de l'Arbre Fruitier (INRA-Université), 4, rue Ledru, 63038 Clermont-Ferrand Cedex, France;(2) Laboratoire de Biologie Cellulaire, C.N.R.S., URA 360, 24, Avenue des Landais, 63177 Aubière Cedex, France |
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Abstract: | A cDNA clone (Bc329) encoding calmodulin was isolated from a Bryonia cDNA library by screening with cloned Arabidopsis calmodulin cDNA. The cDNA Bc329 was 899 bp full-length clone. The predicted amino acid sequence consists of 149 residues and reveals a high homology with other known plant calmodulins (91 to 99% identity). Genomic southern blot suggests that Bryonia calmodulin is encoded by a single-copy gene. The Bc329 clone was used as a probe to study the expression of calmodulin mRNA after a mechanical stimulus applied on young Bryonia internodes. The steady-state of calmodulin mRNA reached a maximum 30 min after the treatment before it progressively decreased. The role of calcium and calmodulin as second messengers is discussed with regard to environmental changes. |
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Keywords: | Bryonia dioica calmodulin cDNA cloning mRNA induction mechanical stimulus |
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