| 多孔硅Bragg反射镜适配子生物传感器检测心肌肌钙蛋白I |
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| 引用本文： | 曾志鹏,张娟琨,刘珺,王明伟,林青,徐慧静.多孔硅Bragg反射镜适配子生物传感器检测心肌肌钙蛋白I[J].生物加工过程,2012(2):68-72. |
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| 作者姓名： | 曾志鹏 张娟琨 刘珺 王明伟 林青 徐慧静 |
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| 作者单位： | [1]天津科技大学生物工程学院天津市工业微生物重点实验室教育部工业微生物重点实验室,天津300457 [2]天津市产品质量监督检测技术研究院,天津300384 |
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| 基金项目： | 科技部资助项目（2009GJA10047）;天津市科委资助项目（07ZHZHNC04500,09ZCZDSF04200） |
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| 摘 要： | 通过脉冲腐蚀法制备多孔硅Bragg反射镜，将心肌肌钙蛋白I（cTnI）适配子共价固定到多孔硅Bragg反射镜的孔洞中，发现适配子能与cTnI分子特异性结合。定量分析不同浓度的cTnI与适配子结合后多孔硅Bragg反射镜的反射谱峰位的红移情况。结果表明：基于多孔硅Bragg反射镜适配子生物传感器的光学检测具有良好的特异性，且具有免标记及检测时间短等优异性能。传感器的线性检测范围0．05-4nmol／L，最低检测限为0．05nmol／L。
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| 关 键 词： | 适配子 生物传感器 多孔硅 Bragg反射镜 心肌肌钙蛋白I |
Detection of cardiac troponin I based on aptamer biosensor on nano-porous silicon Bragg reflector |
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| ZENG Zhipeng,ZHANG Juankun,LIU Jun,WANG Mingwei,LIN Qing,XU Huijing.Detection of cardiac troponin I based on aptamer biosensor on nano-porous silicon Bragg reflector[J].Chinese Journal of Bioprocess Engineering,2012(2):68-72. |
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| Authors: | ZENG Zhipeng ZHANG Juankun LIU Jun WANG Mingwei LIN Qing XU Huijing |
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| Institution: | 1. Key Laboratory of Industrial Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China; 2. Tianjin Product Quality Inspection Technology Research Institute, Tianjin 300384, China) |
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| Abstract: | Nano-porous silicon Bragg reflectors were fabricated using an electrochemical etching process. Then, the cTnI aptamers by binded with cTnI were immobilized to the porous silicon Bragg reflector surface using a crosslink method. After the binding between aptamers and the cTnI of different concentrations, the reflection spectrum red shifts of the porous silicon Bragg reflector were analyzed quantitatively. The result showed that the porous silicon Bragg reflector aptamer biosensor had high specificity. Furthermore,the pro- cedures were label-free and detection time was reduced. The linear detection range of the sensor was 0. 05 -4 nmol/L, and the lowest detected concentration was 0. 05 nmol/L. |
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| Keywords: | aptamer biosensor porous silicon Bragg reflector cardiac troponin I |
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