| 表面改性SBA-15材料固载猪胰脂肪酶 |
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| 引用本文： | 王伟,朱凯,杨波,胡集成,韩萍芳.表面改性SBA-15材料固载猪胰脂肪酶[J].生物加工过程,2012,10(2):40-44. |
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| 作者姓名： | 王伟 朱凯 杨波 胡集成 韩萍芳 |
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| 作者单位： | 南京工业大学生物与制药工程学院,南京,210009 |
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| 基金项目： | 国家重点基础研究发展计划（973计划）资助项目（2009CB72470） |
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| 摘 要： | 采用试剂y-氯丙基三乙氧基硅烷（cvrEs）对介孔硅材料SBA-15进行表面改性，并通过红外图谱（FT-IR）和N2吸附脱附等温图（BET）对其进行表征。结果表明：改性前原材料的比表面积为460．9m2／g，改性后材料比表面积提高到512．0m2／g。利用改性前和改性后的SBA-15对猪胰脂肪酶进行固载实验，并对实验结果进行比较，发现改性后的SBA-15在脂肪酶活性、pH环境适应性、热耐受性和可操作性都优于改性前的SBA-15，在最优条件下的酶活力提高超过60％。
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| 关 键 词： | 酶 固定化 介孔 分子筛 SBA-15 |
Adsorption and catalytic activity of porcine pancreatic lipase on surface modification SBA-15 mesoporous material |
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| WANG Wei
,
ZHU Kai
,
YANG Bo
,
HU Jicheng
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HAN Pingfang.Adsorption and catalytic activity of porcine pancreatic lipase on surface modification SBA-15 mesoporous material[J].Chinese Journal of Bioprocess Engineering,2012,10(2):40-44. |
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| Authors: | WANG Wei ZHU Kai YANG Bo HU Jicheng HAN Pingfang |
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| Institution: | (College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology,Nanjing 210009, China) |
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| Abstract: | Media for enzyme immobilization were prepared by T-chloropropyl triethoxysilane （CPTES） group. The prepared modifying mesoporous silica SBA-15 with support was characterized with FT-IR and BET method and exhibited by high specific surface area and uniform pore size. Furthermore, unmodified SBA-15 and the modified SBA-C1 materials were used for porcine pancreas lipase immobilization. The re- sults were compared with that of enzyme activity, activity retention and stability. The modified SBA-15 material with CPFES showed good performances for porcine pancreas lipase immobilization. Compared with unmodified SBA-15, the adsorption capacity of SBA-C1 to porcine pancreas lipase was improved more than 60%. |
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| Keywords: | enzyme immobilization mesoporous molecular sieves SBA-15 |
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