Electron microscopical demonstration of adenylate cyclase activity in nervous tissue |
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| Authors: | Leena Rechardt Matti Härkönen |
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| Institution: | (1) Department of Anatomy, University of Helsinki, SF-00170 Helsinki 17;(2) Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, SF-00290 Helsinki 29, Finland |
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| Abstract: | Summary Adenylate cyclase (EC 4.6.1.1) activity stimulated by norepinephrine and dopamine was demonstrated histochemically by electron microscopy in the cerebral cortex and caudate nucleus of the rat. The precipitating agent in the histochemical reaction was cobalt, which was shown biochemically to increase the adenylate cyclase activity. The reaction product was located in the synapses, being contiguous attached to the postsynaptic membrane. It was also located in the plasma membrane of some nerve fibers. Alloxan, the specific inhibitor of adenylate cyclase, inhibited the reaction in the cerebral cortex and caudate nucleus, and haloperidol had a somewhat similar effect in the caudate region.Supported by grants from the Medical Research Council in the Academy of Finland |
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