Decrease of cytochrome c oxidase protein in heart mitochondria of copper-deficient rats |
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Authors: | Luisa Rossi Giovanna Lippe Eliana Marchese Angelo De Martino Irene Mavelli Giuseppe Rotilio Maria R Ciriolo |
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Institution: | (1) Department of Biology, University of Rome "Tor Vergata", Rome, Italy;(2) Department of Biomedical Sciences andTechnologies, University of Udine, Italy;(3) Department of Biomedical Sciences, University of Chieti"G. D'Annunzio", Rome, Italy |
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Abstract: | Copper deficiency has been reported to be associated withdecreased cytochrome c oxidase activity, whichin turn may be responsible for theobserved mitochondrial impairment and cardiac failure. We isolatedmito-chondriafrom hearts of copper-deficient rats: cytochrome c oxidase activity was found to be lowerthan incopper-adequate mitochondria. The residual activity paralleled coppercontent of mitochondria and also corresponded with the heme amount associated with cytochromeaa3. In fact, lower absorption in thea-band region of cytochrome aa3 was foundfor copper-deficient rat heart mitochondria. Gel electrophoresisof protein extractedfrom mitochondrial membranes allowed measurements of protein content of thecomplexes ofoxidative phosphorylation, revealing a lower content of complex IV protein incopper-deficientrat heart mitochondria. The alterations caused by copper deficiency appear to bespecific forcytochrome c oxidase. Changes were not observed for F 0 F 1 ATP synthase activity,for heme contents ofcytochrome c and b, and for protein contents of complexes I, III and V.The present study demonstrates that the alteration of cytochrome c oxidase activityobserved in copper deficiency is due to a diminishedcontent of assembled protein and that shortnessof copper impairs heme insertion into cytochrome c oxidase. |
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Keywords: | copper deficiency Cytochrome c oxidase heart mitochondria rat |
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