Identification of a female-sterile mutation affecting yolk protein 2 in Drosophila melanogaster |
| |
Authors: | Williams J L Saunders R D Bownes M Scott A |
| |
Institution: | (1) Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JR Edinburgh, UK |
| |
Abstract: | Summary The three yolk proteins (YP1, YP2 and YP3) of Drosophila melanogaster are synthesised in the fat body and ovarian follicle cells and selectively accumulated in the developing oocytes to provide a nutrient source for embryogenesis. We have described the phenotype of a temperaturesensitive female-sterile mutant, fs(1) K313, and characterised its yolk proteins. This mutation affects the secretion of YP2 and is the first mutation affecting YP2 to be described. Using genetic and molecular tests we argue that the female-sterile phenotype results, at least in part, from the abnormal secretion of YP2 perturbing the follicle cell secretory pathway in general and thus causing defects in chorion protein secretion. The gene coding for YP2 in fs (1) K313 has been cloned and sequenced. Two amino acid substitutions have been found which probably cause the abnormal secretion of YP2 and the resulting female-sterile phenotype. |
| |
Keywords: | Drosophila Yolk protein protein processing secretion female-sterile mutant DNA sequencing |
本文献已被 PubMed SpringerLink 等数据库收录! |
|