The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells |
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| Authors: | Quoix Nicolas Cheng-Xue Rui Guiot Yves Herrera Pedro L Henquin Jean-Claude Gilon Patrick |
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| Institution: | Unit of Endocrinology and Metabolism UCL 55.30, Faculty of Medicine, Université Catholique de Louvain, Avenue Hippocrate 55, B-1200 Brussels, Belgium. |
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| Abstract: | The control of glucagon secretion by pancreatic alpha-cells is poorly understood, largely because of the difficulty to recognize living alpha-cells. We describe a new mouse model, referred to as GluCre-ROSA26EYFP (or GYY), allowing easy alpha-cell identification because of specific expression of EYFP. GYY mice displayed normal glycemic control during a fasting/refeeding test or intraperitoneal insulin injection. Glucagon secretion by isolated islets was normally inhibited by glucose and stimulated by adrenaline. Ca(2+)](c) responses to arginine, adrenaline, diazoxide and tolbutamide, were similar in GYY and control mice. Hence, this new mouse model is a reliable and powerful tool to specifically study alpha-cells. |
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| Keywords: | [Ca2+]c free cytosolic calcium concentration EYFP enhanced yellow fluorescent protein FACS fluorescence activated cell sorting KATP channel ATP-sensitive K+ channel |
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