Benzoxazinone kanamycin A conjugate. A new fluorescent probe suitable to detect mycoplasmas in cell culture |
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Authors: | M Monsigny P Midoux C Depierreux C Bebear M T Le Bris B Valeur |
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Affiliation: | Département de Biochimie des Glycoconjugués et Lectines endogènes, CNRS INSERM, Orléans, France. |
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Abstract: | The synthesis of a new benzoxazinone derivative suitable to detect early infection of cultured cells with mycoplasmas is described. p-[beta-(7-dimethylamino 1,4-benzoxazin 2-one 3yl)-vinyl]- phenylpropenoic acid was coupled to kanamycin A, an aminoglycoside leading to a cationic fluorescent probe which fluoresces at 600 nm upon excitation at 490 nm. This fluorescent probe is shown to heavily label the glycocallix of all the mycoplasma strains tested which are found to be associated with contaminated cultured cells and to allow an easy and rapid detection of contamination by fluorescence microscopy and flow cytometry. |
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Keywords: | flow cytometry polycation aminoglycoside |
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