PCR-based restriction fragment length polymorphism (RFLP) analysis and serotyping of Campylobacter jejuni isolates from diarrheic patients in China and Japan |
| |
Authors: | Masataka Nishimura Masafumi Nukina Jin Mei Yuan Bao Quan Shen Jian Jun Ma Mitsuhiro Ohta Takahiko Saida Takashi Uchiyama |
| |
Affiliation: | Department of Neurology and Clinical Research Center, Utano National Hospital, Ukyo-ku, Kyoto 616, Japan; Public Health Research Institute of Kobe City, Kobe 650, Japan; Department of Neurology, First University Hospital, Beijing Medical University, Beijing 100034, China; Department of Infectious Disease, First University Hospital, Beijing Medical University, Beijing 100034, China; Virus Research Institute, Kyoto University, Kyoto 606, Japan |
| |
Abstract: | Abstract A molecular typing approach for Campylobacter jejuni was applied with restriction fragment length polymorphism (RFLP) analysis of a 702-bp PCR-amplified portion of the flagellin-A ( flaA ) gene. We analyzed a total of 179 strains, including 69 independent clinical isolates from diarrheic patients in Japan, 85 isolates in China, and 25 heat-stable (HS) serotype strains by Penner and Hennessy ((1980) J. Clin. Microbiol. 12, 732–737). Six Afa I, seven Mbo I, and five Hae III RFLPs were found in the 702-bp flaA segment from the 179 strains. Using a combination of these three enzymes, 25 separate RFLP groups were recognized. While 59 of 154 (38.3%) strains obtained in Japan and China were nontypeable by the HS antigenic scheme, all but two of 154 (98.7%) could be typed by RFLP typing. All 11 isolates of HS-19 strains, which are frequently isolated from Guillain-Barré syndrome (GBS) patients, showed an identical RFLP pattern (Cj-1), and Cj-1 consisted only of HS-19 strains. This suggests that the HS-19:Cj-l strain is distinct among C. jejuni strains. This molecular typing method provides a rapid and reliable typing scheme for epidemiological studies of C. jejuni , and may also be useful for the analysis of C. jejuni subtypes from GBS patients. |
| |
Keywords: | Campylobacter jejuni Restriction fragment length polymorphism Heat-stable serotyping Guillain-Barré syndrome Flagellin |
|
|