An enzymatic fluorometric assay for L-lysine in plasma and tissue |
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| Authors: | J M Tiller D L Bloxam |
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| Institution: | Reproductive Biology Laboratory, Institute of Obstetrics & Gynaecology, Queen Charlotte''s Maternity Hospital, London W6 OXG, United Kingdom |
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| Abstract: | A simple, specific, and reliable method has been developed for the determination of L-lysine in blood plasma and tissue. The L-lysine in the sample is decarboxylated enzymatically, and fluorescamine is added to a pentan-1-ol extract of the cadaverine formed. This produces a stable product which is measured fluorometrically. |
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| Keywords: | lysine measurement amino acids fluorescamine fluorometry |
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