Metabolic flux and robustness analysis of glycerol metabolism in <Emphasis Type="Italic">Klebsiella pneumoniae</Emphasis> |
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| Authors: | Qingrui Zhang Hu Teng Yaqin Sun Zhilong Xiu Anping Zeng |
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| Institution: | (1) Department of Bioscience and Biotechnology, Dalian University of Technology, Linggong Road 2, Dalian, 116023, People’s Republic of China;(2) Department of Chemical Engineering, Qingdao University of Science and Technology, Zhengzhou Road 53, Qingdao, 266042, People’s Republic of China;(3) Institute of Bioprocess and Biosystems Engineering, Technical University Hamburg-Harburg, Denickestr. 15, 21071 Hamburg, Germany |
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| Abstract: | The knowledge of the mechanism of flux distribution will benefit understanding cell physiology and regulation of metabolism.
In this study, the measured fluxes obtained under steady-state conditions were used to estimate intracellular fluxes and identify
the robustness of branch points of the anaerobic glycerol metabolism in Klebsiella pneumoniae for the production of 1,3-propanediol by metabolic flux analysis. The biomass concentration increased as NADH2/NAD+ decreased at low initial concentration and inversed at high initial glycerol concentration. The flux distribution revealed
that the branch points of glycerol and dihydroxyacetonephosphate were rigid to the environmental conditions. However, the
pyruvate and acetyl coenzyme A metabolisms gave cells the flexibility to regulate the energy and intermediate fluxes under
various environmental conditions. Additionly, it was found that the formation rate of ethanol and the ratio of pyruvate dehydrogenase
to pyruvate formate lyase appeared visible fluctuations at high glycerol uptake rate. |
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| Keywords: | 1 3-Propanediol Klebsiella pneumoniae Metabolic flux analysis Glycerol metabolism Metabolic network |
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