Chemical characterization of the O-specific chain of Sphaerotilus natans ATCC 13338 lipopolysaccharide |
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Authors: | Hussein Masoud Andràs Neszmelyi Hubert Mayer |
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Affiliation: | 1. Max-Planck-Institut für Immunbiologie, Stübeweg 51, W-7800, Freiburg, Federal Republic of Germany 2. Central Research Institute for Chemistry of the Hungarian Academy of Sciences, Budapest, Hungary
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Abstract: | The chemical structure of the O-specific chain of the Sphaerotilus natans lipopolysaccharide was established. The isolated polysaccharide moiety contained neutral sugars (rhamnose, glucose, and l-glycero-d-manno-heptose), 3-deoxy-d-manno-octulosonic acid (Kdo), phosphate and ethanolamine. Alditol acetate and methylation analyses showed that the O-specific chain contained linear pentasaccharide repeating units, composed of four units of rhamnose, substituted at 3-position, and one unit of glucose, substituted at 4-position. Oxidation by chromium trioxide showed that all sugars were α-linked. The structure proposed for the O-specific chain has been confirmed by periodate oxidation, and by 1H-and 13C-NMR spectroscopic studies for the original and the Smith-degraded PS moiety. The O-specific unit of the S. natans LPS has the following structure: $$begin{gathered} [ to 4) - Glup - (1xrightarrow{a}3) - Rhap - (1xrightarrow{a}3) - Rhap - hfill - (1xrightarrow{a}3) - Rhap - (1xrightarrow{a}3) - Rhap - (1]_n xrightarrow{a} hfill end{gathered} $$ |
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