Chloride dependence of the K+-stimulated release of taurine from synaptosomes |
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Authors: | R. Sánchez Olea H. Pasantes-Morales |
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Affiliation: | (1) Institute of Cellular Physiology, National University of Mexico, Mexico City, Mexico;(2) Instituto de Fisiologia Celular, UNAM, Apartado Postal 70-600., 04510 Mexico, D. F., Mexico |
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Abstract: | Exposure of a crude synaptosomal fraction to K+ concentrations ranging from 25 to 100 mM evokes the release of [3H]taurine and [3H]GABA. These high concentrations of K+ induce, besides depolarization, a marked synaptosomal swelling, which is prevented by replacing chloride in the solutions with the largely impermeant anion gluconate. The depolarizing effect of K+ is unaffected by omission of chloride. The K+-evoked release of taurine seems related to K+-induced changes in synaptosomal volume rather than to a depolarizing effect, since it is totally calcium-independent but is abolished by reducing chloride and by making solutions hypertonic with mannitol. The release of [3H]GABA, in contrast is unaffected in chloride-free or hypertonic solutions. |
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Keywords: | Taurine synaptosome swelling volume-sensitive release chloride-dependent release |
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