Male Killing and Incomplete Inheritance of a Novel <Emphasis Type="Italic">Spiroplasma</Emphasis> in the Moth <Emphasis Type="Italic">Ostrinia zaguliaevi</Emphasis> |
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Authors: | Jun Tabata Yuuki Hattori Hironori Sakamoto Fumiko Yukuhiro Takeshi Fujii Soichi Kugimiya Atsushi Mochizuki Yukio Ishikawa Daisuke Kageyama |
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Institution: | (1) Biodiversity Division, National Institute for Agro-Environmental Sciences, Tsukuba, Japan;(2) Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;(3) Insect-Microbe Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba Ibaraki, 305-8634, Japan; |
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Abstract: | Bacteria of the genus Spiroplasma are widely found in plants and arthropods. Some of the maternally transmitted Spiroplasma endosymbionts in arthropods are known to kill young male hosts (male killing). Here, we describe a new case of Spiroplasma-induced male killing in a moth, Ostrinia zaguliaevi. The all-female trait caused by Spiroplasma was maternally inherited for more than 11 generations but was spontaneously lost in several lineages. Antibiotic treatment
eliminated the Spiroplasma infection and restored the 1:1 sex ratio. The survival rates and presence/absence of the W chromosome in the embryonic and
larval stages of O. zaguliaevi showed that males were selectively killed, exclusively during late embryogenesis in all-female broods. Based on phylogenetic
analyses of 16S rRNA, dnaA and rpoB gene sequences, the causative bacteria were identified as Spiroplasma belonging to the tick symbiont Spiroplasma ixodetis clade. Electron microscopy confirmed bacterial structures in the follicle cells and follicular sheath of adult females. Although
many congeneric Ostrinia moths harbor another sex ratio-distorting bacterium (Wolbachia), only O. zaguliaevi harbors Spiroplasma. |
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