Hydroxynitrile Lyase Catalysis in Ionic Liquid-containing Systems |
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Authors: | Wen-Yong Lou Ruo Xu Min-Hua Zong |
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Institution: | (1) Department of Biotechnology, South China University of Technology, 510640 Guangzhou, P.R. China |
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Abstract: | The cleavage of mandelonitrile catalysed by hydroxynitrile lyases (HNL) from Prunus amygdalus (PaHNL) and Manihot esculenta (MeHNL) proceeded more rapidly in monophasic aqueous media containing 1-propyl-3-methylimidazolium tetrafluoroborate C4MIm]BF4] than in media containing acetonitrile or THF. Both HNLs were much more thermostable in C4MIm]BF4] than in acetonitrile or THF. The addition of each of the four ionic liquids 1-butyl-, 1-pentyl- and 1-hexyl-3-methylimidazolium
tetrafluoroborates at 2–6% (v/v in the aqueous phase) increased both the enzyme activity and the product e.e. in the PaHNL-catalysed transcyanation in an aqueous/DIPE biphasic system. However, MeHNL was inactivated by the ionic liquids,
as indicated by the decreased reaction rate, substrate conversion and product e.e. |
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Keywords: | biocatalysis chiral cyanohydrin hydroxynitrile lyase ionic liquid transcyanation |
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