UV-sensitive mutants of Chlamydomonas reinhardi |
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| Authors: | D R Davies |
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| Affiliation: | 1. Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos Street, GR-11855 Athens, Greece;2. Computational Biology & Medicine Group, Biomedical Research Foundation, Academy of Athens, Soranou Efessiou 4, Athens 11527, Greece;3. Department of Informatics and Telecommunications, National and Kapodistrian University of Athens, University Campus, Athens 15784, Greece;4. Turku Centre for Biotechnology, BioCity, University of Turku and Åbo Akademi University, Turku 20521, Finland |
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| Abstract: | Mutant strains of Chlamydomonas reinhardi have been induced by nitrosoguanidine which are highly sensitive to UV radiation. This enhanced response is due to a defective dark repair capacity. The effect of photoreactivating light is not dose-modifying in the mutants, but a comparison of the doses required to reduce the survival level to 10%, gave photoreactivating factors as large as 45 in some of the mutants, whereas in the parental wild type strain it is 4.7. By crossing mutants with each other and with the wild type, it was shown that the differences in UV response are due to single gene differences, and that several loci are involved. Two of the loci studied have been shown to influence survival in different ways—the double mutant being more sensitive than either parent. |
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