An improvement of cucumber cotyledon greening bioassay for cytokinins |
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| Authors: | Xuejun Yu Gaixia Li Dan Xu Xueliang Dong Xiuxiang Qi Youquan Deng |
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| Institution: | (1) Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, 730000 Lanzhou, P.R.China;(2) Henan Key Laboratory of Fine Chemicals, 450002 Zhengzhou, P.R.China;(3) University of Henan, 475001 Kaifeng, P.R.China |
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| Abstract: | The cucumber cotyledon greening bioassay for cytokinins was modified by using 95% acetone-ethanol instead of 80% acetone as
extraction solvent. The cotyledons were extracted directly with a 2:1 (v/v) acetone-ethanol solution in dark for 24 hours,
omitting the homogenization and centrifugation operations of the previous bioassay. The modified bioassay is more convenient
and especially useful in screening cytokinin-active substance from a large number of samples. |
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| Keywords: | cytokinins cucumber cotyledon greening bioassay chlorophyll extraction 6-benzyladenine N N′ -diphenylurea |
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