Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres |
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| Authors: | Nagaki Kiyotaka Talbert Paul B Zhong Cathy Xiaoyan Dawe R Kelly Henikoff Steven Jiang Jiming |
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| Institution: | Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, USA. |
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| Abstract: | The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis. |
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