Influence of culture conditions on glutathione production by <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Lucielen Oliveira Santos Tatiane Araujo Gonzales Beatriz Torsani Úbeda Ranulfo Monte Alegre |
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Institution: | 1.Department of Food Engineering, College of Food Engineering,UNICAMP (State University of Campinas),Campinas,Brazil |
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Abstract: | A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD)
were carried out with the aim to obtain the best conditions of temperature (20–30°C), agitation rate (100–300 rpm), initial
pH (5.0–7.0), inoculum concentration (5–15%), and glucose concentration (30–70 g/l) for glutathione (GSH) production in shake-flask
culture by Saccharomyces cerevisiae ATCC 7754. By a FFD (25–2), the agitation rate, temperature, and pH were found to be significant factors for GSH production. In CCRD (22) was obtained a second-order model equation, and the percent of variation explained by the model was 95%. The results showed
that the optimal culture conditions were agitation rate, 300 rpm; temperature, 20°C; initial pH, 5; glucose, 54 g/l; and inoculum
concentration, 5%. The highest GSH concentration (154.5 mg/l) was obtained after 72 h of fermentation. |
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Keywords: | Glutathione Saccharomyces cerevisiae Culture optimization Experimental design |
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