| 抗人胃癌单链抗体的分离纯化及其活性测定 |
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| 引用本文： | 张淑梅 李晶. 抗人胃癌单链抗体的分离纯化及其活性测定[J]. 生物技术, 1999, 9(4): 9-11 |
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| 作者姓名： | 张淑梅 李晶 |
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| 作者单位： | 黑龙江省科学院应用微生物研究所 |
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| 摘 要： | 用已经构建的工程菌高效表达抗人胃癌链缺本并使用ＳｅｐｈａｄｅｘＧ－１００柱层析分离纯化抗体蛋白。同时用免疫竞争抑制实验，测出单链抗体具有特异性结合胃癌抗原活性。
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| 关 键 词： | 胃癌 单链抗体 分离纯化 免疫竞争抑制 |
PURIFICATION AND ACTIVITY ASSAY OF SCFV ANTIBODY OF ANTI HUMAN STOMACH CANCER |
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| Zhang Shumei Li Jing Zhao XiaoXiang Zhang Yunhu Li Rongping Yang Zhixing. PURIFICATION AND ACTIVITY ASSAY OF SCFV ANTIBODY OF ANTI HUMAN STOMACH CANCER[J]. Biotechnology, 1999, 9(4): 9-11 |
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| Authors: | Zhang Shumei Li Jing Zhao XiaoXiang Zhang Yunhu Li Rongping Yang Zhixing |
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| Abstract: | e have expressed efficiently ScFv antibody of antihuman stomach cancer in constructed engineering strains. ScFv antibody were purified with SephadexG-100 gel-filtration chromatography, its binding activities against the stomach carcinoma cells were detected by immunological competing inhibition method. |
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| Keywords: | human stomach cancer SCFV antibody purification |
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