| 构建胆固醇氧化酶的短杆菌基因文库 |
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| 引用本文： | 季文明,张和春.构建胆固醇氧化酶的短杆菌基因文库[J].生物技术,1999,9(6):4-8. |
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| 作者姓名： | 季文明 张和春 |
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| 作者单位： | 无锡轻工大学生物工程学院!无锡，214036，无锡轻工大学生物工程学院!无锡，214036，无锡轻工大学生物工程学院!无锡，214036 |
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| 基金项目： | 国家轻工局轻工科技发展计划项目!(G0 3 - 13 ) |
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| 摘 要： | 以内切酶Sau3AI部分水解产胆固醇氧化酶短杆菌的染色体DNA,以质粒pUC18为载体,JM109为宿主,采用碱性磷酸酯酶CIP去除内切酶BamHI水解后质粒的5’端磷酸根,提高质粒的重组率,构建了供筛选胆固醇氧化酶基因的基因文库
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| 关 键 词： | 基因文库 胆固醇氧化酶 甾短杆菌 |
CONSTRUCTION OF BREVIBACTERIUM GENOMIC LIBRARY FOR CHOLESTEROL OXIDASE PRODUCTION |
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| Ji Wenming,Zhang Hechun,Wang Wu.CONSTRUCTION OF BREVIBACTERIUM GENOMIC LIBRARY FOR CHOLESTEROL OXIDASE PRODUCTION[J].Biotechnology,1999,9(6):4-8. |
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| Authors: | Ji Wenming Zhang Hechun Wang Wu |
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| Abstract: | The chromosomal DNA from a Brevibacteium producing the cholesterol oxidase was partial digested by the restriction enzyme Sau3AI,ligated with the plasmid pUC18 digested by BamHI and removed off the 5'-phosphate groups by CIP,the recombinant plasmids was transformed to the host of JM109 and the genomic library for screening the cholesterol oxidase.Gene was constructed. |
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| Keywords: | genomic library cholesterol oxidase Brevibacterium |
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