Measurement of kinase activation in single mammalian cells |
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Authors: | Meredith G D Sims C E Soughayer J S Allbritton N L |
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Institution: | Department of Physiology and Biophysics, University of California, Irvine, CA 92697-4560, USA. |
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Abstract: | We demonstrate a new method for the simultaneous measurement of the activation of key regulatory enzymes within single cells. To illustrate the capabilities of the technique, the activation of protein kinase C (PKC), protein kinase A (PKA), calcium-calmodulin activated kinase II (CamKII), and cdc2 protein kinase (cdc2K) was measured in response to both pharmacological or physiological stimuli. This assay strategy should be applicable to a broad range of intracellular enzymes, including phosphatases, proteases, nucleases, and other kinases. |
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