Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies |
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Authors: | B Thomas S E Penn G W Butcher G Galfre |
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Institution: | (1) Plant Physiology Department, Glasshouse Crops Research Institute, Littlehampton, West Sussex, UK;(2) Monoclonal Antibody Centre, Agricultural Research Council, Institute of Animal Physiology, Babraham, Cambridge, UK |
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Abstract: | A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.Abbreviations ELISA
enzyme-linked immunosorbent assay
- FR
far-red light
- McAb
monoclonal antibody(ies)
- PBS
phosphate-buffered saline
- Pfr
far-red-absorbing form of phytochrome
- Pr
red-absorbing form of phytochrome
- R
red light
- PMSF
phenylmethylsulphonylfluoride |
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Keywords: | Avena (phytochrome) Immunological discrimination Monoclonal antibody Phytochrome (red- far-red absorbing forms) |
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