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The spatial arrangement of germ line cells in ovarian follicles of the mutantdicephalic inDrosophila melanogaster
Authors:Anne Frey  Klaus Sander  Herwig Gutzeit
Affiliation:(1) Biologisches Institut I (Zoologie), Albertstraße 21 a, D-7800 Freiburg, Federal Republic of Germany
Abstract:Summary The pattern of intercellular connections between germ line cells has been studied in follicles of the mutantdicephalic (dic), which possess nurse cell clusters at both poles. Staining of follicles with a fluorescent rhodamine conjugate of phalloidin reveals ring canals and cell membranes and thus allows us to reconstruct the spatial organization of the follicle. Each germ line cell can be identified by the pattern of cell-cell connections which reflect the mitotic history of individual cells in the 16-cell cluster. The results indicate that in both wild-type anddicephalic cystocyte clusters one of the two cells with four ring canals normally becomes the pro-oocyte. However, in some follicles (dicephalic and wild-type) oocytes were found with fewer or more than four ring canals. Indic follicles, one or several nurse cells may become disconnected from the other cells during oocyte growth at stage 9–10. Such disconnected cells cannot later on empty their cytoplasm into the oocyte. This, in turn, might be of consequence for the determination of axial polarity of the embryo.
Keywords:Drosophila  Oogenesis  Ring canals  Oocyte determination  Polarity
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