Isolation and characterization of the haemocytes of Calliphora vicina on density gradients of Ficoll |
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Authors: | P.W. Peake |
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Affiliation: | School of Biological Sciences, University of Sydney, Sydney, 2006, Australia |
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Abstract: | Discontinuous gradients of Ficoll have been used in an equilibrium density analysis of the haemocytes of Calliphora vicina. Using histochemical criteria, it was shown that the acid phosphatase-containing haemocytes decreased in mean density during larval life. Enzymatic analysis, and an analysis of the density distribution of labelled haemocytes at various times after an injection of [H3]-thymidine, provided evidence that a dense, replicating population of cells had been separated from a non-replicating acid phosphatase-containing population. The latter gained increasing amounts of the lysosomal enzymes acid phosphatase and protease as they aged. |
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Keywords: | Insects haemocytes Ficoll density gradient lysosomal enzymes |
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