| 酸胁迫对干酪乳杆菌H+-ATP酶基因表达的影响 |
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| 引用本文： | 陈霞,孙志宏,张文弈,孟和,张和平. 酸胁迫对干酪乳杆菌H+-ATP酶基因表达的影响[J]. 微生物学通报, 2007, 34(3): 0479-0482 |
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| 作者姓名： | 陈霞 孙志宏 张文弈 孟和 张和平 |
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| 作者单位： | 1. 内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特,010018
2. 上海交通大学农业与生物学院,上海,200240 |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 耐酸性是乳酸菌重要的益生菌性状之一。实验采用半定量RT-PCR法分别对在不同酸度条件下培养后的3株不同干酪乳杆菌的H -ATP酶基因mRNA表达水平进行了比较和分析。实验结果显示,随着培养基酸度增加干酪乳杆菌的生长受到抑制,特别在pH4.0条件下干酪乳杆菌的生长受到强烈的抑制;H -ATP酶基因的表达量随着培养基酸度的增加而增加。推测H -ATP酶与干酪乳杆菌耐受酸性条件是有一定的关联的。
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| 关 键 词： | 干酪乳杆菌 耐酸性 H -ATP酶 半定量RT-PCR |
| 文章编号： | 0253-2654（2007）03-0479-04 |
| 修稿时间： | 2006-08-07 |
Expression of H+-ATPase Gene Associated with Different AcidicCondition in Lactobacillus casei |
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| CHEN Xi,SUN Zhi-Hong,ZHANG Wen-Yi,MENG He and ZHANG He-Ping. Expression of H+-ATPase Gene Associated with Different AcidicCondition in Lactobacillus casei[J]. Microbiology China, 2007, 34(3): 0479-0482 |
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| Authors: | CHEN Xi SUN Zhi-Hong ZHANG Wen-Yi MENG He ZHANG He-Ping |
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| Affiliation: | 1. Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018; 2. College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240 |
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| Abstract: | The ability of acid tolerance is one of the most important properties of lactic acid bacteria.To investigate the gene expression of three stains of Lactobacillus casei which incubated in different acidic condition,a semi-quantitative RT-PCR method was used to detect the H -ATPase gene expression in different acidic condition.The result indicated that the growth of Lactobacillus casei had been restrained by the acid stress,especially when the incubated condition changed to pH4.0,and the expression of H -ATPase gene was increased along with the increased of incubated condition acidity.We guess it has some relationship between H -ATPase and the growth ability of Lactobacillus casei in different acidic condition. |
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| Keywords: | Lactobacillus casei acid tolerance H -ATPase Semi-quantitative RT-PCR |
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